Fast Creosote Bioremediation Results

Creosote bioremediation was recently demonstrated by a user of a custom blend of CL Solutions organisms.  The environmental consulting firm applied the microbes to finely shredded wood that had been saturated with creosote.  The initial testing results after bioaugmentation showed a reduction of the creosote concentration by  57% in just 7 days.  The concentration measured as total recoverable hydrocarbons decreased from 3,453 mg/kg to 1,497 mg/kg.

In-Situ Aerobic Soil and Ground Water Bioremediation of PCE and TCE

CL-Out bioremediation of chlorinated solvents like PCE and TCE is an aerobic cometabolic process.  As these compounds are not directly metabolized under aerobic conditions, the competitive advantage goes to the CL-Out organisms that cometabolize them.  While utilizing a sugar substrate, the CL-Out organisms produce enzymes mono- and dioxygenase that bridge the carbon bond to destabilize the compound.  The solvent is converted to an organic acid that the organism can metabolize.  The benefit is that CL-Out bioremediation can be applied to soils both in situ and ex situ.  Click here for to download a case study.  For further explanation call CL Solutions at 513-284-9540.

In-situ Bioremediation of BTEX, TPH and PAHs in Groundwater

Convenience Store in Florida

After three underground storage tanks were removed from the location of a convenience store, investigation showed that petroleum contamination was present in small area near the former dispenser island.  The vadose-zone contaminated soil was excavation and disposed off-site.  Ground water monitoring showed that the ground water impact was limited to a small area near the source of contamination. The decision was made to close the site by applying Petrox® bioremediation to the ground water.

Ground Water Contamination

The ground water contamination was in a shallow water table aquifer that had a medium permeability.  The contamination was a suite of BTEX, PAHs, methylnaphthalene isomers and TRPH.


A pilot study was completed to determine feasibility of Petrox bioremediation combined with a peroxide oxygen supplement.  The pilot study reduced contaminant concentrations by up to 92%.  A full-scale application completed the site remediation.


The pilot study and full-scale applications of Petrox and an oxygen supplement reduced BTEX compounds by >99%, naphthalene by 99%, TRPH by 96% and naphthalene isomers by 88% to achieve the remediation goals and site closure.  The microbial cost for the pilot study and full-scale treatment was less than $2,000.

To learn more, click here to download the case study.

CL-Out Bioaugmentation Following ISCO to Remediate TCE

CL-Out bioremediation was used to remediate ground water at an active manufacturing plant in eastern Massachusetts after ISCO treatment of TCE in groundwater reached a limit.  CL-Out was selected because the aquifer was naturally aerobic.  CL-Out cometabolizes TCE and other chlorinated solvents under aerobic conditions by producing an oxygenase enzyme that breaks the carbon to carbon bond.  Under aerobic conditions daughter products such as DCE and vinyl chloride are not formed.  30 days after one application of CL-Out the TCE concentration was reduced by 97% and the site remediation goals were achieved. Click here to download the case study.

Oil-Contaminated Water Treatment

CL Solutions provides a special consortium of microbes for remediation of water containing high concentrations of used or waste oil.  Under various conditions, the oil removal rate was as much as 1,300 mg/L/day.  In some situations the remediation goal is simply to remove the visible sheen or separate-phase oil.  With aeration, CL Solutions microbes were able to remove the visible oil layer and reduce the TPH concentration from 29% to less than 1% in 30 days at a waste oil lagoon. For more information and other case studies click here.

A special consortium of microbes removed the visible oil layer from this wastewater.