Photographs of Field Applications of Bioaugmentation

These photographs show actual field applications of CL-Out and Petrox.

CL-Out and Petrox are typically packaged in drum liners for convenience.

The hydrated microbes are staged typically for 12 to 24 hours prior to application.

Hydrated microbes are transfered from the drum to the contaminated media for treatment.

CL-Out and Petrox are provided in bulk bags for hydration in tanks larger than 55 gallons.

Hydrated micorbes may be applied to the surface and blended into contaminated soil.

The hydrated microbes may be injected into soil or ground water for in situ treatment.

Blending in the micorbes can also increase available oxygen.

 

What is MEOR?

MEOR is microbial enhanced oil recovery.  Some microbes produce biosurfactants that increase the water-solubility of oil.  The biosurfactants’ benefit microbes by making it easier for the microbes to metabolize oil.  Additionally, the biosurfactants can strip oil from rock and soil surfaces to improve environmental remediation by extraction, air stripping, or sparging.

The Pseudomonas sp. in Petrox are naturally occuring petroleum-degrading microbes with the broadest metabolic capabilities.  Their production of biosurfactants is one of their features that makes them so effective.  Pseudomonas sp. produce both glycolipid and lipopeptide surfactants.

An initial increase in concentrations may be observed after Petrox is used at a site due to the biosurfactants.  However, the concentrations decrease as the microbes metabolize the oil.  This feature has been used to remove oil safely from inaccessible or sensitive locations such as near utilities, tanks or building structures.

Micorbial Insights, Inc. (micorbe.com) has biosurfactant assays available t0 test for the presence of the genes in Pseudomonas sp. and other organisms that produce biosurfactants.  If tests show that they are abscent from a site, you can enhance the oil recovery with Petrox and the surfactants it produces.

In addition to the biosurfactants that Petrox produces, a non-ionic surfactant is added to Petrox to formulate Petrox EC.  This combination makes Petrox EC a strong cleaning agent to remove oil and grease from surfaces or contaminated subsurface soil or rock.

CL Solutions continues to expand the use of biological products such as Petrox and Petrox EC for bioremediation and microbial enhanced oil recovery.

Bioremediation of Phthalates

Bioremediation of phthalatescan reduce risks to human health and the environment.  Phthalates are a  a family of common industrial chemicals used in plastics and other consume products.  Phthalates can damage the liver, kidneys, lungs and reproductive system.  Petrox microbes can remove these contaminants from water or soil to reduce potential exposure to these risks.

Petrox bioremediation of phthalates has been demonstrated in field and laboratory studies to remove phthalates from soil and ground water.  Field application of Petrox bioremediation reduced bis(2-ethylhexyl)phthalate (BEP) concentrations from 650 ppm to 397  ppm in soil and 300 to 39 ppb in ground water at a site in Rochester, New York.  At the same site di-n-octylphthalate (DOP) in soil was reduced from 7.5 to 1.9 ppm. Click here to view the case study.

Oil-Contaminated Water Treatment

CL Solutions provides a special consortium of microbes for oil-contaminated water treatment.   Under various conditions, the oil removal rate was as much as 1,300 mg/L/day.  In some situations the remediation goal is simply to remove the visible sheen or separate-phase oil.  With aeration, CL Solutions microbes were able to remove the visible oil layer and reduce the TPH concentration from 29% to less than 1% in 30 days at a waste oil lagoon. For more information and other case studies click here.

A special consortium of microbes removed the visible oil layer from this wastewater.

Pesticide Bioremediation – Landfarming Application for DDD, DDE and Toxaphene

Petrox® bioremediation was used to remediate pesticide-contaminated soil at an industrial location in California.

Soil Remediation

The soil contamination consisted of pesticides including 4,4-DDD, 4’4-DDE and toxaphene.  Soil samples were sent to CL Solutions for bench-scale treatability studies.   The following table shows the  maximum detected concentrations and the treatment results.

Contaminant
Untreated Concentrations (ug/Kg)
Post-treatment concentrations (ug/Kg)
4,4-DDD
390
250
4,4-DDE
380
220
Toxaphene
8,500
440

After the bench-scale verification, full-scale bioremediation was implemented.  The contaminated soil was placed in two stockpiles and treated with Petrox. The following table shows the pre- and post-treatment results.

Contaminant Concentrations (ug/Kg)
Contaminant
Pile 1
Pile 2
Before
After
Before
After
4,4-DDD
310
170
14
nd ( <5)
4,4-DDE
810
300
8.7
nd  (<5)
Toxaphene
12,000
7,300
380
nd (<5)

Conclusions

The Petrox treatment successfully reduced the concentrations of pesticide contaminants in the soil stockpiles.  For additional information or assessment of applicability to your site contact CL Solutions.