PAH Bioremediation

The results of a recent bench-scale test confirmed the effectiveness of a special blend of CL Solutions’ microbes formulated for PAH bioremediation.  The test results showed that after two weeks, the total concentration of 15 PAH compounds decreased by 85% from a total of 358 mg/kg to 50.9 mg/kg.  Benzo(A)pyrene is often a most difficult PAH to remove. The test showed that the custom blend reduced the benzo(A)pyrene concentration from 24.5 mg/kg to 3.68 mg/kg.  Tests will continue to determine whether even greater effectiveness can be achieved over 30 days.

In-Situ Petroleum Bioremediation Rates With Petrox

Recent data from field applications of Petrox bioremediation show degradation rates of 100 to 500 micrograms per liter (ug/ml) per day. These degradation rates are for total petroleum hydrocarbons or total BTEX, depending on the site monitoring requirements.

Achieving high degradation rates requires high initial concentrations. Initial concentrations of 1,000 to 10,000 ug/l were used to develop these degradation rates.

At lower concentrations, the degradation rates depend on effective distribution of microbes for cell to contaminant contact. Secondly, desorption of petroleum constituents from submerged soil may cause rebound and suggest lower degradation rates. For these reasons we cannot calculate a degradation rate for petroleum at low concentrations.

In situ Heavy Oil Bioremediation in Alberta

Using a special microbial blend, Delta Environmental has been successfully bioremediating high concentrations of heavy oil in soil around pipelines and oil production equipment.  At a typical site, Delta reduced the concentration of petroleum C34 to >C50 from 130,000 mg/Kg to less than 10,900 in 30 days.  That is a removal rate of 3,970 mg/Kg/day.  This approach not only benefits the environment and meets green cleanup standards, but saves money and equipment downtime for the facility.

Bioremediation of Naphthalene and Methylnaphthalene Isomers

Petrox bioremediation accelerates the removal of naphthalene and isomers of methylnaphthalene.  These petroleum compounds are often slow to remediate due to their low volatility and strong affinity for absorption onto soil particles.  The remediation rate of these compounds is slower than BTEX compounds.  They often also rebound as the absorbed phase solubilizes following removal of the compound from ground water to reestablish phase equilibrium.  Petrox bioremediation accelerates the remediation of these compounds by a combination of metabolizing the dissolved phase and a biosurfactant effect that strips the compound from soil to make it available for metabolism.

For example, Petrox achieved more than 90% removal of these compound in less than 9 months at a site of a former underground storage tank (UST) in Florida.  After the UST and surrounding contaminated soil were removed, the residual BTEX compounds were treated by chemical oxidation.  The chemical oxidation, however, did not remove the naphthalene and methylnaphthalene isomers. Petrox was applied to the ground water in and around the former UST cavity.

Nine months after the Petrox application the following reductions in concentrations were achieved.  The concentrations are the average of four ground water sampling locations.

Naphthalene –  367 ug/L reduced to 18 ug/L,  95% removal.

1 Methylnaphthalene – 255 ug/L reduced to 33 ug/L, 87% removal.

2 Methylnaphthalene – 400 ug/L reduced to 30 ug/L, 93% removal.

The Petrox was injected into the ground through a continuous low-volume injection system.  This application method did not impact the use of the property or require major construction. The application was also far less costly than alternatives.

The greatest benefit was the relatively rapid removal of difficult contaminants.

 

 

 

 

 

 

The Basics of Petroleum Bioremediation

For a brief summary of the basics of petroleum bioremediation follow this link Basics of Petroleum Bioremediation