Petrox microbe thrive on naphthalene and are very efficient at naphthalene bioremediation in the field. In fact, naphthalene is occasionally used in the QA/QC process to verify Petrox viability and effectiveness. In this test, the Petrox organisms are placed on an agar devoid of a carbon source. The naphthalene is applied to the top plate as the sole carbon source. The Petrox viability is then demonstrated by colony growth on the top plate as shown on the following picture.
With Petrox microbes, in situ TPH bioremediation reduced the concentration of petroleum in soil at a former gas station from 9,500 mg/kg to 52 mg/kg. The impacted soil was at more than 20 feet deep. Injection of calcium peroxide provided the oxygen needed for metabolism of the petroleum by Petrox microbes. The post treatment sampling was 90 days after the bioaugmentation, so that even though the average remediation rate was 105 mg/kg/day, the peak remediation rate must have been much higher when the Petrox population was higher.
This is another example of fast, reliable bioremediation by Petrox organisms.
In situ bioremediation reduced ground water contamination by more than 99% in less than a year at a gas station in Florida.
Convenience Store in Florida
After three underground storage tanks were removed from the location of a convenience store, investigation showed that petroleum contamination was present in small area near the former dispenser island. The vadose-zone contaminated soil was excavation and disposed off-site. Ground water monitoring showed that the ground water impact was limited to a small area near the source of contamination. The decision was made to close the site by applying Petrox® bioremediation to the ground water.
Ground Water Contamination
The ground water contamination was in a shallow water table aquifer that had a medium permeability. The contamination was a suite of BTEX, PAHs, methylnaphthalene isomers and TRPH.
A pilot study was completed to determine feasibility of Petrox bioremediation combined with a peroxide oxygen supplement. The pilot study reduced contaminant concentrations by up to 92%. A full-scale application completed the site remediation.
The pilot study and full-scale applications of Petrox and an oxygen supplement reduced BTEX compounds by >99%, naphthalene by 99%, TRPH by 96% and naphthalene isomers by 88% to achieve the remediation goals and site closure. The microbial cost for the pilot study and full-scale treatment was less than $2,000.
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In situ remediation is a contact sport, requiring contact between the microbes and contamination. Clay-rich soils are particularly challenging as the low permeability limits effective distribution of inoculants. While numerous closely-spaced injection points can improve the distribution of inoculants, this approach is often not possible at active properties and can be costly. Horizontal drilling was used on the subject site to overcome the combined challenges of low permeability and limited site access to inject Petrox® microbes for bioaugmentation. The combined technologies reduced the total BTEX concentrations in ground water from 2,771 µg/L to 645 µg/L in less than one year.
The site is a former gas station located in the Florida panhandle. The site soils are mixed silt and clayey silts that are typical of the coastal setting. The depth to the water table varied seasonally from 13 to 18 below ground surface.
The underground storage tanks and contaminated soil had been removed prior to the ground water treatment. Residual ground water contamination exceeded Florida Ground Water Cleanup Target Levels (GCTLs) for benzene, toluene, ethylbenzene, xylene, naphthalene and methylnaphthalene isomers.
The proposed treatment area was approximately 2,500 square feet. The treatment depth was from 10 to 25 feet below grade. The treatment depth included the capillary fringe to treat residual contamination above the seasonal low water table.
Access to the treatment area was limited by the current property use, the proximity of roads on two sides and a building on the third. Horizontal drilling was selected by the site consultant, Advanced Environmental Technologies (www.aetllc.com) to deliver Petrox® to the contamination.
The horizontal drill rig was set back from the treatment zone on the opposite side of the building. The horizontal injection wells were set in four horizontal sets of eight wells. The horizontal layers were at 10, 15, 20 and 25 feet deep. The wells in each layer were five feet apart. A total of 32 injection wells were closely spaces for excellent coverage through the treatment zone.
Petrox® was injected into the ground water in two treatment events – November 6, 2008 and June 24, 2009. Petrox® was delivered in each injection well as the drill stem was withdrawn through the treatment zone. The injection was monitored for accuracy so that 0.2 gallons of Petrox® was injected per foot of injection zone. A total of 320 gallons of Petrox® slurry was injected.
Following the Petrox® injection, air was injected periodically through vertical sparging wells to increase the oxygen availability for the microbial metabolism.
Ground water samples were collected from a monitoring well inside the treatment area to track the progress of the bioremediation. Approximately 60 days after the first Petrox® treatment, analysis of ground water samples showed 84% reduction in the benzene concentration and 35% reduction in the total BTEX compounds concentrations. There was an apparent increase in the xylene concentration due to ground water mixing and induced increase in solubility due to the bioaugmentation injections.
A second sampling event approximately 30 days after the second injection showed additional reduction in the contaminant concentrations. After the second injection, the total BTEX concentrations were 23% of the original concentration with xylene decreasing from 1,200 to 95 µg/L.
In addition to the BTEX compounds, naphthalene and methylnaphthalene isomer concentrations decreased through both treatments.
Horizontal drilling and injection made it possible to remediate ground water at this site of petroleum contamination with limited access and low natural permeability. Without disturbing the property use, the horizontal injection of Petrox® provided effective distribution of the microbes for bioremediation. The injection may have also made the contamination more available for bioremediation by increasing the contaminant solubility as shown by temporary increases in concentration.
This case study demonstrates that in situ bioaugmentation may be a feasible solution for sites with limited permeability and access restrictions. For more information contact CL Solutions at www.cl-solutions.com.
The goal of bioaugmentation is to improve the rate of contaminant removal by adding a high population of beneficial microbes to the contaminated media. The additional microbes should provide short-term benefit as the microbes begin metabolizing the contaminants immediately upon injection. But what benefit does bioaugmentation provide in the long term? And how much benefit does bioaugmentation provide over biostimulation by adding nutrients to the native organisms?
A client of CL Solutions completed a bench-scale study to answer these questions. A bench-scale study was preferred to a field study because it removes the potential distribution and time-lag issues associated with the distances between injection and monitoring locations in the field.
Samples of petroleum-contaminated soils were obtained and separated into split samples for treatment with microbes and nutrients. Some were untreated for comparison. Samples were tested for petroleum concentrations, including C-fraction concentrations after 30, 40 and 60 days. Heterotrophic populations were measured at 40 and 60 days.
The tests showed the following results in the early stages:
- All of the treated samples showed more than 80% total petroleum reduction in the first 30 days.
- The sample treated with nutrients only had the same level of petroleum removal as the bioaugmented samples in the first 30 days.
- The heterotrophic population of the biostimulated sample was as high as in the bioaugmented samples at 40 days.
After 30 days the situation changed.
- The bioaugmented microbial population continued to increase after 40 days and may have increased by a factor of 100 times. Meanwhile, the biostimulated population appeared to stall.
- The petroleum removal continued in the bioaugmented samples and reached as high as 93% removal. In comparison the biostimulated sample stalled at 82% removal.
- The difference appears to be that the bioaugmented samples removed the C-21 to C-35 concentrations at a much higher rate than the biostimulated sample.
- Phenanthrene was target chemical for bioremediation. The biostimulated sample showed 39% removal while the bioaugmented samples showed complete removal to BDL.
Overall, the superior performance of the bioaugmented samples appears to be related to having a greater metabolic range that removed the heavier hydrocarbon fractions. Microbes with the extended metabolic range could continue to multiply as they grew on the heavy hydrocarbon fraction. The results are consistent with field results showing the recalcitrance of heavier hydrocarbon fractions and compounds like naphthalene and phenanthrene under natural attenuation.
Contact CL Solutions for more information and insights.