California Site Closure with Petrox Bioremediation

Southern California Gasoline UST Release Site

After removal of USTs, piping and surrounding soils, ground water was treated with 110 gallons of Petrox® by direct injection in December 2009.  Post-treatment sampling shows that the bioaugmentation reduced the contaminant levels to below regulatory standards by the next semi-annual sampling round.

Sampling Date

TPHg Benzene Toluene Ethylbenzene

Total   Xylenes

MTBE
Pre-bioaugmentation

10/6/09

1,200 320 7.2 700

70

5
Post-bioaugmentation

4/26/10

<50 <0.5 <0.51 <1

<5

<0.5

All concentrations shown in µg/L.

Florida Site Closures With One Petrox Application

The following applications demonstrate the significant results that can be achieved with a single application of Petrox bioremediation.

Florida Panhandle Gas Station

Facility ID # 32-8520334

Petrox was injected through horizontal drilling to address a ground water plume with limited surface access.  After a single Petrox® application there was significant reduction in the contaminant concentrations.

Sampling Date Benzene Toluene Ethyl benzene Xylene Naph-thalene 2-Methyl naphthalene
Pre- bioaugmentation 11/11/05 4,300 3,000 1,300 5,300 240 48
10/23/07 2,700 4,200 860 5,700 240 41
Post-bioaugmentation 11/06/08 1.7 8.5 0.71 5.1 0.49 0.45
1/22/09 0.88 0.64 0.43 1.3 0.49 0.45

All Concentrations shown in µg/L.

 

Southeast Florida Gas Station

Facility ID 8838788

After removal of USTs, piping and surrounding soils Ground water was treated with 110 gallons of Petrox® by direct injection in June 2009.  Sampling six months after the Petrox® treatment showed significant reductions in all contaminants.

Sampling Date Benzene Toluene Ethylbenzene Total Xylenes
Pre-  bioaugmentation 4/4/2008 480 1800.0 110.0 900.0
Post- bioaugmentation 11/12/09 <0.5 <0.51 <0.44 <0.5

All concentrations shown in µg/L.

 

Southeastern Florida Marina

Tequesta, Florida

Facility ID # 43-8731703

Subsequent to the removal of petroleum underground storage tanks (USTs), piping and surrounding soils for redevelopment, residual soil and ground water contamination at the site were treated with Petrox® bioremediation.  Petrox® was applied to the soil that was removed during the UST removals and to the ground water in the UST cavity.

Approximately 4,000 tons of soil were removed from the excavation and stockpiled on site for treatment.  The total BTEX concentrations in the excavated soil were up to 100 mg/kg.  Petrox® bioremediation was applied to the stockpiled soils.  In less than 90 days the petroleum was no longer detected in the soil and in the soil met the applicable Florida DEP standards.

Petrox® bioremediation was applied to the water in the former UST cavity to treat residual petroleum.  The proximity of the cavity to the Jupiter Sound indicated that the ground water was strongly influenced by infiltration from the Sound.  After less than 90 days post treatment, the water in the excavation also met the applicable Florida DEP standards.

 

Maximum Water Conc. Benzene Toluene Ethylbenzene Total Xylenes
Pre-treatment 1,960 5,140 1,860 18,340
90 days after treatment 3.7 BDL BDL BDL

All concentrations shown in µg/L. BDL= below detection limits.

 

Central Florida Gas Station

Petrox was applied to the ground water in an area around a single monitoring well that did not respond to another treatment as well as the rest of the plume.  A single 10-gallon application of Petrox® was sufficient to apply for site closure.

Sampling Date Benzene Toluene Ethylbenzene Total Xylenes
Pre- treatment 8/2/2007 11,100 1,250 1,260 5,040
Post-treatment 10/4/07 1,600 20.0 290. 840.
11/19/07 0.40 1.0 1.00 32.0

All concentrations shown in µg/L.

 

Fast, Low-Cost Petroleum Spill Treatment

After removal of USTs, ground water was treated over a one-half acre plume in Houston, Texas.  Petrox bioaugmentation was implemented by injection of 110 gallons of high concentration microbial solution in April 2006.  Post-treatment sampling shows the bioaugmentation significantly reduced the source area contaminant levels  at a cost of less than $2,000 for the Petrox®.

Sampling Date Benzene Toluene Ethylbenzene Total    Xylenes
Pre-  bioaugmentation 4/4/06 2,000 1,400 1,600 2,460
Post- bioaugmentation 8/9/06 350 240 470 69

All concentrations shown in µg/L.

LNAPL Removal with Petrox

Site Summary

Petrox® bioaugmentation was implemented to remediate fuel spilled at a railway in at Central, Ohio.  The impacted soil in the source area was excavated for off-site disposal.  The fuel, however, percolated through the fill into underlying soil.  The fuel migrated through the soil, seeped to the surface and formed a sheen on the water of an adjacent pond.  The LNAPL was removed and no longer visible in the pond and ground water monitoring seeps in less than 90 days after one application of Petrox®.

Geology and Hydrogeology

The spill occurred on a railroad embankment that is adjacent to a pond. The fill of the embankment is 2 to 3 feet thick.  The fill is on top of sandy clay deposits.  The slope of the embankment is very steep and slopes down to the pond.  After the fuel began to appear on the surface water, sumps were installed near the base of the slope and perched ground water was encountered at a few feet deep in the sumps.  The remediation was based on a conceptual model for the fuel seepage and migration.  Under the conceptual model, the fuel percolated through the fill to the native silty clay soil.  Once in the native soil, the fuel migrated through fractures as an LNAPL and seeped from weeps in the hillside.

Contamination

In immediate response to the fuel spill, the impacted ballast and fill were excavated and disposed off site.  Residual fuel remained in the soil below the excavation.  A hydrocarbon sheen was observed on the surface of the adjoining surface water approximately one month after the spill.  Containment booms were placed on the surface water and sumps were installed in the adjoining slope.  The LNAPL on ground water was as much as 0.2 feet thick in the sumps. The assumed area of the LNAPL was approximately 1,500 square feet.

Remediation

Petrox® was applied by injecting one drum (55-gallons) of a concentrated solution into the fill in the excavation area.  The Petrox® was allowed to flush through the impacted soil and follow the migration path of the fuel.  As the weather became seasonally dry, additional water was added to the excavation to support the microbial growth and to flush the microbes into the soil fractures.  During remediation the LNAPL thicknesses were measured in the sumps.  After each measurement the LNAPL was bailed from the sumps.

Results

Following Petrox® bioaugmentation the LNAPL thickness appeared to increase in some locations.  However, in 90 days the LNAPL was no longer present in the sumps and a sheen was no longer observed on the surface water.  The following table shows representative LNAPL thickness measurements (in feet).

Sump Number Pre-Treatment 30 Days Post Treatment 60 Days Post Treatment 90 Days Post Treatment
1 0.04 0.06 No LNAPL No LNAPL
10 0.15 0.12 0.08 No LNAPL
11 0.13 0.08 0.07 No LNAPL
15 0.12 0.10 0.18 No LNAPL

 

The treatment was successful in reducing the residual environmental impact of the fuel spill.  The treatment also verified the effectiveness of Petrox® bioaugmentation for petroleum LNAPL situations

Fast Dry Cleaner Bioremediation

Fast dry cleaner bioremediation by CL-Out microbes reduced PCE concentrations in ground water by more than 99% in less than 90 days to achieve GCTL standards at a dry cleaners in Florida.   The naturally aerobic aquifer conditions supported this aerobic cometabolism of PCE.

It is observed at many sites that naturally aerobic conditions prevent the natural reduction of PCE and TCE.  This is obvious when the suite of contaminants include high concentrations of PCE or TCE without daughter products.  Instead of working against the natural conditions to drive the aquifer conditions anaerobic to facilitate reductive dechlorination, a more sustainable approach is to use CL-Out aerobic cometabolism to remove the parent compounds without producing daughter products.  This approach has been used at hundreds of sites such as this example in Florida.

Contact CL Solutions for more information about this approach.