Bioaugmentation results can be accelerated and amplified by soil mixing to improve distribution and contact. The equipment shown in this example was used to blend Petrox with contaminated soil to a depth of 10 feet.
One of the benefits of CL-Out cometabolism is that the enzymes produced by the Pseudomonas sp. organisms convert a wide range of organic chemicals into organic acids that can be metabolized. The aggressive application of CL-Out organisms to soil and ground water at a chemical mixing company removed the source of ground water contamination by chloroethanes and chloroethenes, including PCE and 1,1,1-TCA. The aerobic cometabolism of these compounds achieved MCLs in less than 6 months. Click here to view the case study Mixed Solvent Plume Cometabolism by CL-Out
For a brief summary of the basics of petroleum bioremediation follow this link Basics of Petroleum Bioremediation
Aerobic cometabolism of TCE may be limited by the potential microbial toxicity of by products or by product metabolic repression. An independent comparison of induced cometabolism using soluble methane and CL-Out bioaugmentation showed that CL-Out bioaugmentation removed more TCE. The TCE removal rate by CL-Out when supplemented with an oxygen source was a steady rate that continued past apparent limits of induced cometabolism. The significant difference may be in that the population of beneficial microbes that can be added is much higher than the population level that may be achieved through biostimulation. For a brief summary of the study follow this link Overcoming TCE Metabolic Limits.
Petrox bioremediation is used to remove petroleum contamination from soil and water under many different conditions. From surface spills to deep soil and ground water contamination, Petrox has successfully removed the contamination and environmental risk. This summary of case studies demonstrates the applicability of Petrox bioremediation to the full range and life cycle of petroleum spills. Click here to view the document.