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Aerobic Cometabolism of Chlorinated Solvents

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CL-Out® aerobic cometabolism successfully remediated solvents in ground water at an operating industrial site in central New Jersey.  The manufacturing and plating facility had historical petroleum and solvent releases.

Chlorinated solvents found in the ground water in two plumes covered a combined area of about one half acre.  A mix of chlorinated solvents including 1,1,1-TCA, 1,1-DCE and PCE in low concentrations icontaminated the ground water.    The remediation goal was to reduce the mass of contamination in the ground water plume to prevent mitigate off-site risks.

After the CL-Out aerobic cometabolism, ground water concentrations in all monitoring wells decreased by more than 80%, which was sufficient to meet the remediation standards.  Click here to download a complete case study.

CL-Out Bioaugmentation Following ISCO to Remediate TCE

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Bioremediation used to complete closure

CL-Out bioremediation was used to remediate ground water at an active manufacturing plant in eastern Massachusetts after ISCO treatment of TCE in groundwater reached a limit.  CL-Out was selected because the aquifer was naturally aerobic.  CL-Out cometabolizes TCE and other chlorinated solvents under aerobic conditions by producing an oxygenase enzyme that breaks the carbon to carbon bond.  Under aerobic conditions daughter products such as DCE and vinyl chloride are not formed. Thirty days after one application of CL-Out the TCE concentration was reduced by 97% and the site remediation goals were achieved. Click here to download the case study.

Pesticide Bioremediation – Landfarming Application for DDD, DDE and Toxaphene

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Petrox® bioremediation was used to remediate pesticide-contaminated soil at an industrial location in California.

Soil Remediation

The soil contamination consisted of pesticides including 4,4-DDD, 4’4-DDE and toxaphene.  Soil samples were sent to CL Solutions for bench-scale treatability studies.   The following table shows the  maximum detected concentrations and the treatment results.

Contaminant
Untreated Concentrations (ug/Kg)
Post-treatment concentrations (ug/Kg)
4,4-DDD
390
250
4,4-DDE
380
220
Toxaphene
8,500
440

After the bench-scale verification, full-scale bioremediation was implemented.  The contaminated soil was placed in two stockpiles and treated with Petrox. The following table shows the pre- and post-treatment results.
Contaminant Concentrations (ug/Kg)
Contaminant
Pile 1
Pile 2
Before
After
Before
After
4,4-DDD
310
170
14
nd ( <5)
4,4-DDE
810
300
8.7
nd  (<5)
Toxaphene
12,000
7,300
380
nd (<5)

Conclusions

The Petrox treatment successfully reduced the concentrations of pesticide contaminants in the soil stockpiles.  For additional information or assessment of applicability to your site contact CL Solutions.

In Situ CL-Out Bioremediation of Industrial Solvents

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Case Study :

Industrial Manufacturing Site, Illinois

Remediation Summary

Leaks from an aboveground solvent tank impacted soil and ground water quality at a manufacturing site in Illinois.  The impact was found under the adjoining building as well as the area near the tank.  The soil and ground water were treated in situ with CL-Out® bioremediation microbes.  Through cometabolism CL-Out® microbes reduced the contaminant concentrations to acceptable levels in less than one year.

Contaminants Soil Results (mg/Kg) Ground Water Results (mg/L)
Pre-Treatment Post- Treatment Pre-Treatment Post- Treatment
PCE 41.8 1.69 5.59 0.006
TCE 4,670 632 15.6 0.026
Cis 1,2-DCE 171 56.6 7.43 0.029
Vinyl Chloride BDL BDL 0.095 0.013

Implementation and Results

Soil Type:  Silty clay till

Treatment Area:  15,000 sq. ft.

Unsaturated soil thickness:  16 ft.

Saturated aquifer thickness:  5 ft.

Treatment: Two applications, initial treatment with 13 units of CL-Out® and follow up treatment with 5 units.

Product Cost: $30,000

Conclusions

CL-Out® bioremediation quickly and cost-effectively reduced the contaminant concentrations to acceptable levels.  Through cometabolism the parent and daughter products were removed simultaneously.  CL-Out® bioaugmentation accelerated the site remediation and reduced uncertainty by applying the right microbes where they were needed.

Surface Oil Spill Bioremediation

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Petrox microbes provide quick response surface oil spill bioremediation.  Petrox can be applied to the oil surface with a backpack sprayer to initiate rapid bioremediation.  This approach protects surface water and vegetation with minimal surface traffic.

In the following example, an oil spill affected the vegetation and surface water at the edge of a lake. Contractors applied Petrox to the oil surface on vegetation and soil.  Periodic soil samples tracked the results.  After 90 days, the maximum and average concentrations decreased by more than 95% at a very low cost.

Surface Spill Results