In Situ Bioremediation of TPH with Petrox

Petrox reduced the concentration of TPH in soil at a former gas station from 9,500 mg/kg to 52 mg/kg. The impacted soil was at more than 20 feet deep.  Injection of calcium peroxide provided the oxygen needed for metabolism of the petroleum by Petrox microbes.  The post treatment sampling was 90 days after the bioaugmentation, so that even though the average remediation rate was 105 mg/kg/day, the peak remediation rate must have been much higher when the Petrox population was higher.

This is another example of fast, reliable bioremediation by Petrox organisms.

In-Situ Aerobic Soil and Ground Water Bioremediation of PCE and TCE

CL-Out bioremediation of chlorinated solvents like PCE and TCE is an aerobic cometabolic process.  As these compounds are not directly metabolized under aerobic conditions, the competitive advantage goes to the CL-Out organisms that cometabolize them.  While utilizing a sugar substrate, the CL-Out organisms produce enzymes mono- and dioxygenase that bridge the carbon bond to destabilize the compound.  The solvent is converted to an organic acid that the organism can metabolize.  The benefit is that CL-Out bioremediation can be applied to soils both in situ and ex situ.  Click here for to download a case study.  For further explanation call CL Solutions at 513-284-9540.

In-situ Bioremediation of BTEX, TPH and PAHs in Groundwater

Convenience Store in Florida

After three underground storage tanks were removed from the location of a convenience store, investigation showed that petroleum contamination was present in small area near the former dispenser island.  The vadose-zone contaminated soil was excavation and disposed off-site.  Ground water monitoring showed that the ground water impact was limited to a small area near the source of contamination. The decision was made to close the site by applying Petrox® bioremediation to the ground water.

Ground Water Contamination

The ground water contamination was in a shallow water table aquifer that had a medium permeability.  The contamination was a suite of BTEX, PAHs, methylnaphthalene isomers and TRPH.


A pilot study was completed to determine feasibility of Petrox bioremediation combined with a peroxide oxygen supplement.  The pilot study reduced contaminant concentrations by up to 92%.  A full-scale application completed the site remediation.


The pilot study and full-scale applications of Petrox and an oxygen supplement reduced BTEX compounds by >99%, naphthalene by 99%, TRPH by 96% and naphthalene isomers by 88% to achieve the remediation goals and site closure.  The microbial cost for the pilot study and full-scale treatment was less than $2,000.

To learn more, click here to download the case study.

Oil-Contaminated Water Treatment

CL Solutions provides a special consortium of microbes for remediation of water containing high concentrations of used or waste oil.  Under various conditions, the oil removal rate was as much as 1,300 mg/L/day.  In some situations the remediation goal is simply to remove the visible sheen or separate-phase oil.  With aeration, CL Solutions microbes were able to remove the visible oil layer and reduce the TPH concentration from 29% to less than 1% in 30 days at a waste oil lagoon. For more information and other case studies click here.

A special consortium of microbes removed the visible oil layer from this wastewater.

Benzo(a)pyrene Bioremediation Using Custom Blend Microbes

Benzo(a)pyrene is a difficult remediation challenge. It is resistant to biological and chemical destruction and extraction from soil.  CL Solutions Custom Blend microbes, however, have successfully remediated benzo(a)pyrene and other PAHs in soil during ex situ land farming treatment.  By placing the contaminated  material in windrows, controlling the temperature and moisture, the addition of Custom Blend microbes has reduced the benzo(a)pyrene concentration by as much as 330 ug/kg/day.

The same approach works on high as well as low concentrations.  At a site in Oregon, the benzo(a)pyrene concentration was reduced from 140 ug/Kg to below detection limits in 7 days.  At a site in Ohio, the benzo(a)pyrene concentration was reduced from 28,000 ug/kg to 3.8 ug/Kg in 60 days.  At both sites, Custom Blend microbes effectively treated the full suite of PAHs along with the benzo(a)pyrene.