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1,1,1-TCA Bioremediation at an Industrial Site in Dayton, Ohio

Fast, Economical Site Cleanup

CL-Out bioremediation reduced the concentration of 1,1,1-TCA in ground water at a steel manufacturing facility in Dayton, Ohio. Following implementation of ozone treatment and high vacuum extraction for more than 2 years, the contaminant concentrations remained high.  Bioaugmentation followed up on these treatments and reduced the 1,1,1-TCA concentration very quickly.  Within 30 days of bioaugmentation, the source area contaminant concentration decreased by 80%.  After a second application, the concentration decreased from the pre-treatment concentration of 1,100 ug/L to 1.4 ug/L.  The bioaugmentation effect was observed as far as 250 feet downgradient where the concentrations decreased by more than 50%.  Click here for the full case study.

 

 

Bioremediation of a Dry Cleaners

Cost-effective Bioremediation of a Dry Cleaners Quickly Removed Contamination

Historical operations at a Houston area dry cleaners resulted in a chlorinated solvent plume (perchloroethylene or PCE) in the shallow ground water. Permeable shallow soil at the site allowed rapid vertical migration at the source and lateral migration in the shallow ground water. This migration resulted in an off-site migration of the plume beneath an apartment complex causing concern for potential vapor intrusion hazards; as well as, a decrease in property value.

A ground-water extraction and treatment system was installed to address the
dissolved phase concentrations. The system reduced the total volatile organic compound
(VOC) concentration in the source area from 115,400 to 36,500 µg/l. However, over
time, operating maintenance and cost escalations were exceeding the on-going remedial
benefit. The system was shut down after nine years of operation.

A review of available remedial technologies that could be implemented within the physical constraints of the
site was conducted. In-situ enhanced aerobic bioremediation was selected to address the
remaining ground-water concentrations because of the permeability of the formation, the
relatively aerobic conditions, and the benefit of reducing the risk of VC accumulation.  Click here to view the aerobic cometabolic degradation pathway.

Click here for a link to the full report from the 2007 Battelle Conference on Bioremediation

 

Brownfield Plan Included CL-Out Bioremediation

A brownfield plan included Cl-Out bioremediation to reduce high concentrations of TCE and other contaminants in ground water at an industrial site near Newark, New Jersey. Using a dynamic remediation plan, three applications of CL-Out microbes followed remediation progress sampling during the site preparation for construction. After reducing the maximum CVOC concentrations from over 8,000 ug/L to less than 20 ug/L, the site met the remediation goals and received a No Further Action Letter from NJDEP. For more information and a detailed case study, click here.

In Situ TPH Bioremediation with Petrox

With Petrox microbes, in situ TPH bioremediation reduced the concentration of petroleum in soil at a former gas station from 9,500 mg/kg to 52 mg/kg. The impacted soil was at more than 20 feet deep.  Injection of calcium peroxide provided the oxygen needed for metabolism of the petroleum by Petrox microbes.  The post treatment sampling was 90 days after the bioaugmentation, so that even though the average remediation rate was 105 mg/kg/day, the peak remediation rate must have been much higher when the Petrox population was higher.

This is another example of fast, reliable bioremediation by Petrox organisms.

What enzymes Are Involved in Aerobic Bioremediation of PCE and TCE?

CL-Out aerobic bioremediation PCE and TCE is a sustainable process for soil and ground water remediation.  As these compounds are not directly metabolized under aerobic conditions, the competitive advantage goes to the CL-Out organisms that cometabolize them.  While utilizing a sugar substrate, the CL-Out organisms produce  mono- and dioxygenase  enzymes that bridge the carbon bond to destabilize the compound.   The solvent is converted to an organic acid that the organism can metabolize.  View the degradation pathway.  The benefit is that CL-Out bioremediation can be applied to soils both in situ and ex situ.  Click here for to download a case study.  For further explanation call CL Solutions at 513-284-5940.