Confidential Site with excess nitrate in ground water

Nitrogen is one of the elements required for microbial cell growth. Excess nitrate in ground water, however, can be harmful. Denitrification is the process by which select organisms can convert nitrate into nitrogen gas. Bacteria that mediate denitrification, utilize nitrate as a metabolic terminal electron acceptor under anaerobic conditions. While Petrox® normally metabolizes organic compounds using oxygen as an electron acceptor, the organisms in Petrox® DN can utilize the same metabolic pathway to remove excess nitrate from ground water under aerobic or anaerobic conditions.

Petrox® DN is a consortium of Pseudomonas putida, which are well known for their metabolic diversity. These naturally-occurring organisms are non-pathogenic and are manufactured under food-grade protocols. Petrox® DN contains a guaranteed cell count of 109 cfu/ml. The high population of denitrifying organisms accelerates the removal of nitrate and removes the guesswork from project management.

Laboratory Validation

A bench-scale treatability study demonstrated the high rate of denitrification by Petrox® DN. At a population of 107 cfu/mL, Petrox® DN removed nitrate at a rate of 17.1 mg/L/hr under anaerobic conditions.


Field Validation

Petrox® DN was applied to nitrate contamination in ground water two locations. In field application 1, after two monthly treatments, Petrox® DN reduced the nitrate concentration by 65%. In the second field application, after just one Petrox® DN application the bioremediation removed 50% of the nitrate in 56 days.

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Petrox® DN is provided freeze-dried for ease of use. Once hydrated Petrox® DN can be introduced into the ground water through injection points or well. Once in the aquifer a carbon source is added to support the population growth. Under favorable conditions the organisms will flourish with a high capacity to remove nitrate, until the nitrate is depleted. For additional information contact CL Solutions at 513-475-6625.