A CL-Out bioremediation push-pull pilot study was implemented at a confidential dry cleaners site to verify the applicability of CL-Out bioremediation to the site and to determine whether the availability of oxygen to support cometabolism of the halogenated solvents would limit the bioremediations.
Geology and Hydrology
Although the impacted ground water was relatively shallow, the site geology was complicated and varied across even this small site. In general the glacial deposits at the site were mainly till with interbedded sandy and silty zones. The impacted ground water was contained within the granular deposits.
The main ground water contaminant was PCE with lower concentrations of TCE, cis -1,2 DCE and vinyl chloride. In the pilot study area, the PCE concentration was 44,200 μg/L and the total of the daughter products was 14,750 μg/L.
Pilot Study Design
A pilot study was completed in the source area in the fall of 2009. One unit of CL-Out was injected into the affected ground water in the source area. One unit of CL-Out is a 55-gallon slurry with a microbial concentration of 109 cfu/ml. The CL-Out microbes were injected with 50 pounds of dextrose to provide a carbon source to support the energy requirements of the population. Pre- and post -treatment samples were taken on February 19 and December 10, 2009. The post-treatment sample was taken after the monitoring well was purged of the injected volume. The following table shows the contaminant concentration trends in the treatment area.
The pilot study verified the applicability of CL-Out bioremediation to the site. The total concentration of CVOCs was reduced from 59,000 to 13,740 μg/L. The CL-Out bioremediation was most effective in the removal of PCE and TCE. There was an increase in vinyl chloride suggesting some incomplete reductive dechlorination, probably by native dehalogenating organisms. Full-scale treatment will be most effective with the addition of an oxygen supplement to limit the dehalogenation and promote the cometabolism of the daughter products. Overall the push-pull pilot study verified the effectiveness of CL-Out bioremediation and provided insights for making full-scale application more effective.